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EXPERIMENT AND GUIDE FOR MEDICAL BIOLOGY（医学生物学实验）

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作者：侯燕芝著

出版社：高等教育出版社

出版时间：2012 年1月

I S B N：9787040334333

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医学生物学实验

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EXPERIMENT AND GUIDE FOR MEDICAL BIOLOGY（医学生物学实验）

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Experiment and Guide for Medical Biology

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EXPERIMENT AND GUIDE FOR MEDICAL BIOLOGY（医学生物学实验）

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18.00元

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内容简介

this manual is designed to be used in a course of experiments in medical biology. it will serve mainly the needs of international students of clinical medical major. it will serve any student who wishes to learn both major principles and modern techniques of medical biology.
　　by using this manual, students will develop laboratory skills, become familiar with the methods of science, and learn important concepts of experiments in medical biology. the manual provides students with an opportunity to apply what they have learned in textbook and lectures in an interesting and informative way.
　　the manual is easy for students to understand and use, and it does not require lengthy or elaborate interpretation by an instructor. each experiment includes a precisely stated purpose (objective),an experimental principle, the materials needed, and a procedure with detailed instructions. the illustrations, diagrams, and tables clarify the directions. this saves time in preparing and performing laboratory activities for both students and instructors. the results and questions of each experiment are designed for recording data during anexperiment, for answering questions, and for submitting materials for evaluation.

作者简介

《experiment and guide for medical biology（医学生物学实验）》
chapter 1 introduction to the medical biology laboratory
1.1 objectives of a teaching medical biology laboratory
1.2 laboratory rules
1.3 laboratory safety
1.4 common laboratory equipments
1.5 experimental methods in medical biology
1.6 evaluation of experimental data and laboratory reports
chapter 2 experiments in medical biology
experiment 1 the use of glass pipets and pipettors
experiment 2 ampboteric ionization and isoelectric point determination of proteins
experiment 3 quantitative determination of protein concentrations by coomassie brilliant blue assay
experiment 4 quantitative determination of proteins by folin-phenol assay
experiment 5 determination of proteins by uv absorption
experiment 6 separation of amino acid mixtures by ion-exchange chromatography
experiment 7 separation of serum y-globulins by ion-exchange chromatography
experiment 8 separation of serum proteins by cellulose acetate membrane electrophoresis
experiment 9 separation and purity determination of serum y-globulins by sds-polyacrylamide gel electrophoresis
experiment 10 determination of serum g!utamic-pyruvic transaminase activity
experiment 11 determination of serum urea nitrogen content

.experiment 12 determination of blood glucose concentration
experiment 13 subculture of adhesion cells
experiment 14 counting and determination of live/dead cells
experiment 15 preparation of cell samples for observation of cell morphologies
experiment 16 mitosis and meiosis of cells
experiment 17 lntracellular distribution of biomacromolecules
experiment 18 isolation of subcellular components
experiment 19 preparation of chromosome samples from mouse bone marrow cells
experiment 20 banding pattern of human chromosomes
experiment 21 colony forming efficiency and total protein analysis of tumor cells
experiment 22 isolation, amplification and identification of the b-actin gene fragment from mouse liver cells
experiment 23 purification of target dna and generation of recombinant dna
experiment 24 transformation and screening of recombinant dna
experiment 25 determination of serum proteins using western blotting