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| Preface to thefifih edition xiii List ofcontributors List ofabbreviations 1. General principles of biochemical investigations I. Simpkins 1.1 The nature ofbiochemistry 1.2 Bioenergetics 1.3 Methods for investigating metabolism 1.4 Practical considerations 1.5 In viva models 1.6 In vitro models 1.7 Microscopy 1.8 Keyterms 1.9 Calculations 1.10 Suggestions for further reading 2. Molecular biology and basic techniques R. Rapley 2.1 Introduction 2.2 Components and primary structure of nucleic acids 2.3 Genes and genome complexity 2.4 The nature of the genetic code 2.5 Cellular location ofnucleic acids 2.6 The cellular functions of DNA 2.7 The manipulation ofnucleic acids：basic tools and techniques 2.8 Isolation and separation of nucleic acids 2.9 Restriction mapping of DNA fragments 2.10 Nucleic acid blotting methods 2.11 Gene probe derivation 2.12 Labelling DNA gene probe molecules 2.13 The polymerase chain reaction 2.14 Nucleotide sequencing ofDNA 2.15 Bioinformatics and the Internet 2.16 Key terms 2.17 Calculations 2.18 Suggestions for further reading 3. Recombinant DNA and genetic analysis R. Rapley 3.1 Introduction 3.2 Constructing gene libraries 3.3 Cloning vectors 3.4 Hybridisation and gene probes 3.5 Screening gene libraries 3.6 Applications of gene cloning 3.7 Expression offoreign genes 3.8 Analysing genes and gene expression 3.9 Analysing whole genomes 3.10 Molecularbiotechnology and applications 3.11 Keyterms 3.12 Suggestions for further reading 4. Immunochemical techniques R. Thorpe and S. Thorpe 4.1 Introduction 4.2 Production of antibodies 4.3 Purification and fragmentation ofimmunoglobulins 4.4 Immunoprecipitation 4.5 Labelling antibodies 4.6 Immunoblotting 4.7 Immunoassays 4.8 Immunohisto/cytochemistry 4.9 Affinity and avidity 4.10 Immunochemical use of surface plasmon resonance 4.11 Key terms 5. Centrifugation techniques A. Griffiths 6. Protein structure, purification and characterisation J. Walker 7. Biomolecular interactions I. Enzymes K. Wilson 8. Biomolecular interactions II. Cell surface receptors and transporters K. Wilson 9. Spectroscopic techniques I. Atomic and molecular electronic spectroscopy D. B. Gordon 10. Spectroscopic techniques II. Vibrational spectroscopy and electronic and nuclear spin orientation in magnetic fields D. B. Gordon 11. Mass spectrometric techniques D. B. Gordon 12. Electrophoretic techniques J. M. Walker 13. Chromatographic techniques K. Wilson 14. Radioisotope techniques R. J. Slater 15. Electrochemical techniques P. K. Robinson. |
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