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| 本手册涵盖了关于拟南芥的基本实验方法。著者对这些方法进行了系统的搜集整理和认真审核，对实验过程进行了详尽的分步介绍，并就如何解决常见问题给出了非常有效的建议，可操作性和指导性较强。对于开展植物分子和细胞生物学、植物遗传和发育研究具有较高的参考价值。 |
| Preface, xi CHAPTER 1 How to Grow Arabidopisi, 1 Morphology and Anatomy Seed Collection and Storage Cultivation of Arabidopsis CHAPTER 2 Obtaining Mutants, Obtaining Previously Identified Mutants from the Stock Centers Forward Genetics:Finding Mutations That Cause Particular Phenotypes CHAPTER 3 Genetic Analysis of Mutants, Setting up Crosses Segregation Analysis CHAPTER 4 How to Analyze a Mutant Phenotypically, Growth Parameters Hormone Response Response to the Abiotic Environment Bacterial Pathogens Oomycete Pathogens: Peronospora Parasitica CHAPTER 5 How to Transform Arabidopsis, Vectors and Agrobacterium Hosts Transformation of Agrobacterium In Planta Transformation of Arabidopsis Selectiong Transformed Plants CHAPTER 6 How to Isolate a Gene Defined by a Mutation, Isolating a Gene Defined by an Insertion Mutation TAIL-PCR Isolationg a Gene Known Only by the Phenotypes of Mutant Alleles:Positional Cloning CHAPTER 7 How to Study Gene Expresio |
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