| Part 1 Basic Experiments Experiment 1 Observation of Bacterial Morphology Ⅰ. Using Oil Immersion I.ens of the Microscope Ⅱ. Basic Morphology and Special Structure of Bacteria Experiment 2 Bacterial Staining and Microscopy Ⅰ. Gram Stain Ⅱ. Acid-fast Stain （Ziehl-Neelsen Method） Experiment 3 Preparation of the Basic Medium Experiment 4 Examination Methods of Parasitic Infections Ⅰ. Direct Fecal Smear Method for Detecting Ascaris Eggs Ⅱ. Examination for Ascaris Larvae in Lung Tissue of Mice Ⅲ. Saturated Brine Flotation Method for Detecting Hookworm Eggs Ⅳ. Fecal Culture Method for Hookworm Larvae Ⅴ. Cellophane Tape Method for Detecting Pinworm Eggs Ⅵ. Direct Sputum Smear or Centrifugal Sedimentation Method for Detecting Paragonimus Eggs " Ⅶ. Squash Method for Detecting Striated Muscle Larvae of Trichinella Ⅷ. Test of Concentration of Eggs by Sedimentation for Detecting S. Japonicum Eggs Ⅸ. The Miracidium Hatching Method for Detecting S. Japonicum Ⅹ. Circumoval Precipitation Test （COPT） for Diagnosing Schistosomiasis Ⅺ. Direct Stool Smear with Iodine-stain for Detecting Amoeba Cysts Ⅻ. Blood Smear Method for Detecting Malarial Parasites Experiment 5 Agglutination Reaction Ⅰ. Direct Agglutination Reaction -Identification of the ABO Blood Group System Ⅱ. Indirect Agglutination Reaction -Anti-streptolysin O Test Ⅲ. Indirect Agglutination Inhibition Reaction-Latex Pregnancy Test Experiment 6 Precipitation Reaction Ⅰ. Single Radial Immunodiffusion Test Ⅱ. Double Immunodiffusion Test Experiment 7 Enzyme-linked Immunosorbent Assay （Sandwich ELISA Assay） Part 2 Comprehensive Experiments Unit 1 Isolation of Microorganisms from the Environment and Human Body Experiment 8 Bacteria in the Air and Ultraviolet Radiation Experiment 9 Bacteria on the Finger Tip and Solid Surface Unit 2 Detection of Skin Infections Experiment 10 Cultural Characteristics of Bacteria and Fungi （Teaching Demonstration） Ⅰ. Clostridium Perfringens in the Milk Medium Ⅱ. Fungi in Sabouraud Dextrose Medium Experiment 11 Observation of Plasmodium, Toxoplasma Gondii , Trichinella Spiralis ,Echinococcus Granulosus and Insecta Ⅰ. Plasmodium Ⅱ. Toxoplasma Gondii Ⅲ. Trichinella Spiralis Ⅳ. Echinococcus Granulosus Ⅴ. Insecta Unit 3 Detection of Respiratory Infections Experiment 12 Isolation & Identification of Pyogenic Cocci Ⅰ. Isolation and Culture of Mimic Pus Swab and Throat Swab Ⅱ. Coagulase Test Experiment 13 Cultural Characteristics of Respiratory Infected Bacteria Ⅰ. The Colonies of Pathogenic Cocci on Blood Agar Plates（Teaching Demonstration） Ⅱ. Mycobacterium Tuberculosis in Lowenstein-Jensen Solid Medium（Teaching Demonstration） Experiment 14 Observation of Paragonimus Westermani Unit 4 Detection of Enteric Infections Experiment 15 Isolation and Identification of Pathogenic Enterobacteria Ⅰ. Isolation of Artificial Fecal Specimens Ⅱ. Biochemical Activities of Enteric Pathogens--Kligler Iron Agar Test Ⅲ. Serologic Identification of Enteric Pathogens Experiment 16 Widal Test Experiment 17 Observation of Ascaris lumbricoides, Hookworms,Enterobius Vermicularis,Taenia ,Entamoeba and Cryptosporidium Ⅰ. Ascaris Lurnbricoides Ⅱ. Hookworms （Ancylostoma Duodenale and Necator Americanus ） Ⅲ. Enterobius Vermicularis Ⅳ. Intestinal Cestodes （Taenia Solium and Taenia Saginata） Ⅴ. Intestinal Protozoa （ Entamoeba Histolytica and Entamoeba Coli ） Ⅵ. Cryptosporidium Unit 5 Detection of Hepatic Infections Experiment 18 Observation of Clonorchis Sinensis Experiment 19 Observation of Schistosoma Japonicum Unit 6 Detection of Genitourinary Tract Infections Experiment 20 Observation of Chlamydia Trachomatis Inclusion Unit 7 Antimicrobial Resistance Experiment 21 Bacterial Conjugation:the Transfer of Plasmids Coding for Antibiotic Resistance Experiment 22 Antibiotic Sensitivity Test Unit 8 Immune Cells Function Assay Experiment 23 Macrophage Phagocytosis Assay Experiment 24 Neutrophil Phagocytosis Assay Experiment 25 Plaque Forming Cells Assay （PFC Assay） Experiment 26 Lymphocyte Transformation Assay Experiment 27 Classification of T Cell Subset and FACS Analysis Experiment 28 Cytokine Measurement by ELISPOT Appendix Appendix 1 Commonly Used Dye and Staining Appendix 2 Media Appendix 3 Commonly Used Reagents and Buffers Index |
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