《间充质干细胞在心血管疾病中的应用:从基础到临床(英文版)》由浙江大学出版社出版。 |
Jian'an Wang，MD，PhD，Professor of Cardiology at Zhejiang University and investigator at Loma Linda University，Southern California，has broad expertise in the molecular and cellular biological processes underlying cardiovascular disease.He has won the Chinese Medical Science and Technology Medal of Honor. .. << 查看详细 |
1 mscs isolation and culture ex vivo 1.1 cell isolation 1.1.1 direct adherence method 1.1.2 density gradient centrifugation 1.1.3 magnetic microbead or flow cytometry method 1.2 cell culture 1.2.1 materials 1.2.2 procedures 1.3 cell cryopreservation and thawing 1.3.1 materials 1.3.2 procedures references 2 mscs identification 2.1 minimal criteria 2.2 some comments of the criteria 2.3 other methods 2.3.1 morphology characteristics 2.3.2 other markers 2.4 conclusion .references 3 biological characteristics of mscs 3.1 surface markers and paracrine characteristics 3.1.1 surface markers 3.1.2 paracrine characteristics of mscs 3.2 electrophysiological properties of mscs and their electric coupling with cardiomyocytes 3.2.1 characterization of ion channels in mscs 3.2.2 why does an individual cell express different currents? 3.2.3 electric coupling of mscs with host cardiomyocytes 3.3 proliferation of mscs and telomerase properties 3.3.1 structure and function of telomeres and telomerase 3.3.2 telomere and telomerase in mscs 3.4 multilineage transdifferentiation of mscs 3.4.1 mscs and cardiomyogenesis 3.4.2 differentiation fate of mscs 3.5 immunological characteristics of mscs 3.5.1 immunological characteristics of mscs 3.5.2 in vivo studies 3.5.3 imnmne-related properties of mscs in cardiology... 3.6 conclusion references 4 utilization of mscs for repairing cardiomyocytes 4.1 application of mscs on myocardial infarction 4.2 application of mscs on cardiomyopathy and chronic heart failure 4.3 conclusion references 5 current status of mscs in clinical application 5.1 the type and number of transplanted cells 5.2 cell preparation 5.3 the time point and pathway of cell transplantation 5.4 others 5.5 conclusion references 6 mscs as a vector of gene engineering 6.1 adenoviruses and angiogenesis-related genes 6.2 adeno-associated virus (aav) and anti-inflammation related genes 6.3 retrovirus and anti-apoptosis related genes 6.4 lentivirus and pacemaker current gene 6.5 conclusion references 7 feasibility of mscs transplantaion 7.1 general ethical considerations 7.2 safety issues 7.3 informed consent 7.4 conclusion references 8 status and expectation of mscs therapy 8.1 clinical application and outcomes 8.2 mechanism of therapeutic effects 8.3 expectations with mscs therapy references index |
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