最 低 价：￥15.40
定 价：￥22.00
作 者：YU Hong,Huang XinXing Chief Editors 著
出 版 社：武汉大学出版社
出版时间：2008 年9月
I S B N：9787307065345
| chapter ⅰ spectrophotometry 1.1 basic concepts and principle of spectrophotometry 1.2 measu rement of light absorption 1.3 aplications of spectrophotometry exp.1 quantitative analysis of protein exp.2 determination of blood glucose chapter ⅱ electrophoresis 2.1 basic principles of electrophoresis 2.2 impact factors of electrophoresis 2.3 detection of components after electrophoretic separation 2.4 special electropho retic techniques and applications exp.3 separation of serum proteins by cellulose acetate membrane electrophoresis exp.4 separation of serum lipoproteins by agarose gel electrophoresis exp.5 proteins separation by sds—polyacrylamide gel electrophoresis chapter ⅲ chromatography 3.1 fundamental principles of chromatography 3.2 com monly used chromatog raphic techniques exp.6 detecting transamination of amino acids by paper chromatography exp.7 separation of mixed amino acids by cation exchange chromatography exp.8 separation of hemoglobin and dinitrophenyl(dnp)-glutamate by gel filtration chromatography .chapter ⅳ enzyme analysis 4.1 the activity of enzyme 4.2 enzyme kinetics(factors affecting reaction velocity) 4.3 enzvmarie analysis exp.9 assay the activity of alanine aminotransferase(alt)in serum (mohun’s method) exp.10 lactate dehydrogenase(ldh)analysis exp.11 effect of substrate concentration on enzyme activity.determining km value for alkaline phosphatase exp.12 coupled enzymatic reaction assay(enzymaticendpoint method)-determination of total cholesterol，triglycerides and glucose in serum chapter ⅴ isolation and purification of protein 5.1 methods and basic principles of protein pu rification 5.2 procedure of protein preparation 5.3 crystallization，concentration，drying and storage of protein 5.4 identification and analysis of protein exp.13 isolation and identification of serum igg exp.14 expression.purification and identification of glutathione s.transferase fusion protein chapter ⅵ isolation.purification and identification of nucleic acids 6.1 isolation and purification of nucleic acids 6.2 identification and analysis of nucleic acids exp.15 isolation of eukaryotic genomic dna by proteinase k.phenolor nai method exp.16 sds.alkaline lysis of plasmid dna exp.17 isolation of total rna by trizol reagent …… chapter ⅶ genetic engineering english-chinese index |
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