| Preface List of Contributors Color Plates 1 Expression of Ion Channels in Xenopus Oocytes （Alan LGoldin） 1.1 Introduction 1.2 Advantages and Disadvantages of Xenopus Oocytes 1.3 Procedures for Using Oocytes 1.4 Types of Analyses 1.5 Examples of Use 1.6 Conclusions Acknowledgments References 2 Molecular Biology Techniques for Structure – Function Studies of Ion Channels （Louisa Stevens, Andrew JPowell, and Dennis Wray） 2.1 Introduction 2.2 Methods for cDNA Subcloning 2.3 Generation of Chimeric Channel cDNAs 2.4 Site-directed Mutagenesis 2.5 Epitope-tagged Channels and Fusion Partners 2.6 Channel Subunit Concatamers 2.7 Concluding Remarks References 3 Unnatural Amino Acids as Probes of Ion Channel Structure – Function and Pharmacology （Paul BBennett, Niki Zacharias, John BNicholas, Sue Dee Sahba, Ashutosh Kulkarni, and Mark Nowak） 3.1 Introduction 3.2 Unnatural Amino Acid Mutagenesis Methodology 3.3 Unnatural Amino Acid Mutagenesis for Ion Channel Studies 3.4 Structure–Function Example Studies 3.5 Other Uses of Unnatural Amino Acids as Probes of Protein Structure and Function 3.6 Conclusions Acknowledgements References 4 Functional Expression of Ion Channels in Mammalian Systems （Jeff JClare） 4.1 Introduction 4.2 cDNA Cloning and Manipulation 4.3 Choice of Host Cell Background 4.4 Post-translational Processing of Heterologous Expressed Ion Channels 4.5 Cytotoxicity 4.6 Transient Expression Systems 4.7 Stable Expression of Ion Channels 4.8 Summary Acknowledgements References 5 Analysis of Electrophysiological Data （Michael Pusch） 6 Automated Planar Array Electrophysiology for Ion Channel Research （Derek J Trezise） 7 Ion Flux and Ligand Binding Assays for Analysisof Ion Channels （Georg CTerstappen） 8 Ion Channel Assays Based on Ion and Voltage-sensitive Fluorescent Probes 9 Approaches for Ion Channel Structural Studies （Randal BBass and Robert HSpencer）0 Molecular 10 Modeling and Simulations of Ion Channels: Applications to Potassium Channels Acknowledgements References Subject Index |
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