最 低 价：￥609.80
定 价：￥677.55
作 者：Silas G. Villas-Boas，JensNielsen， JornSmedsgaard， Michael A. E. Hansen， Ute Roessner-Tunali 著 著
出 版 社：Oversea Publishing House
出版时间：2007-2-1
I S B N：9780471743446
| SILAS G. VILLAS-BÔAS, PHD, is a Research Scientist at AgResearch Limited in New Zealand.
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| PREFACE LIST OF CONTRIBUTORS PARTⅠ:CONCEPTS AND METHODOLOGY 1 Metaboiomics in Functional Genomics and Systems Biology 1.1 From genomic sequencing to functional genomics, 1.2 Systems biology and metabolic models, 1.3 Metabolomics, 1.4 Future perspectives, 2 The Chemical Challenge of the Metabolome 2.1 Metabolites and metabolism, 2.2 The structural diversity of metabolites, 2.2.1 The chemical and physical properties, 2.2.2 Metabolite abundance, 2.2.3 Primary and secondary metabolism, 2.3 The number of metabolites in a biological system, 2.4 Controlling rates and levels, 2.4.1 Control by substrate level, 2.4.2 Feedback and feedforward control, 2.4.3 Control by "pathway independent" regulatory molecules, 2.4.4 Allosteric control, 2.4.5 Control by compartmentalization, 2.4.6 The dynamics of the metabolism--the mass flow, 2.4.7 Control by hormones, 2.5 Metabolic channeling or metabolons, 2.6 Metabolites are arranged in networks that are part of a cellular interactome, Sampling and Sample Preparation 3.1 Introduction, 3.2 Quenching--the first step, 3.2.1 Overview on metabolite turnover, 3.2.2 Different methods for quenching, 3.2.3 Quenching microbial and cell cultures, 3.2.4 Quenching plant and animal tissues, 3.3 Obtaining metabolites from biological samples, 3.3.1 Release of intracellular metabolites, 3.3.2 Structure of the cell envelopes--the main barrier to be broken, 3.3.3 Cell disruption methods, 3.3.4 Nonmechanical disruption of cell envelopes, 3.3.5 Mechanical disruption of cell envelopes, 3.4 Metabolites in the extracellular medium, 3.4.1 Metabolites in solution, 3.4.2 Metabolites in the gas phase, 3.5 Improving detection via sample concentration, 4 Analytical Tools 4.1 Introduction, 4.2 Choosing a methodology, 4.3 Starting point--samples, 4.4 Principles of chromatography, 4.4.1 Basics of chromatography, 4.4.2 The cbromatogram and terms in chromatography, 4.5 Chromatographic systems, 4.5.1 Gas chromatography, 4.5.2 HPLC systems, 4.6 Mass spectrometry, 4.6.1 The mass spectrometer--an overview, 4.6.2 GC-MS--the El ion source, 4.6.3 LC-MS--the ESI ion source, 4.6.4 Mass analyzer--the quadrupole, 4.6.5 Mass analyzer--the ion-trap, …… PARTⅡ:CASE STUDIES AND REVIEWS INDEX |
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